The same patient was collected at the same time: the relationship between hsCRP, serum hsCRP, and CRP results?
Director Chen Baorong, Department of Clinical Laboratory, Beijing Aerospace General Hospital: Theoretically, whole blood collected by the same patient at the same time hsCRP, serum hsCRP, CRP should be able to obtain the same measurement results, but in fact, the measurement results of the three projects in most laboratories are usually only consistent, especially at low concentration levels, mainly with measurement methods. Learning is related to the sample type. On the one hand, there are two main types of quantitative measurement methods for CRP in the domestic market: immunoturbidimetric turbidity and immunoturbidimetric turbidity. The measurement principles of these two methods are inconsistent and will inevitably lead to the development of these two types of measurement principles. The important methodological characteristics such as sensitivity, linear range, interference factor, and precision measured by each kit are inconsistent, which ultimately leads to differences in measurement results. On the other hand, the whole blood is different from the serum sample type. Although the CRP is used to identify the sample in the immunological principle, whether the non-serum component still present in the whole blood sample interferes with the measurement and the cell is dissolved by the whole blood sample. Whether the sample produced after filtration or not is completely equivalent to the serum component is still confirmed by more research data.
Du Hongxin, Department of Biochemistry, Chongqing Three Gorges Central Hospital:CRP is a kind of synthesis by the liver and can be associated with S. pneumoniae C polysaccharide Reacts APRP. The main biological function of CRP is to activate the complement and mononuclear phagocytic system by binding to ligands (apoptotic and necrotic cells, or phosphorylcholines such as human invading bacteria, fungi, parasites, etc.). Pathogen or pathological cell clearance of the ligand. When the body is infected, tissue damage and inflammatory diseases, CRP rises rapidly, can be thousands of times, and its content drops rapidly after the disease is cured. Therefore, CRP testing can provide valuable information for the diagnosis, treatment and monitoring of inflammatory processes and related diseases. The CRP detection methods mainly include an immunoturbidimetric method and an immunoturbidimetric method. In recent years, based on the immunoturbidimetric method, the detection of high-sensitivity C-reactive protein (hs-CRP) has been developed. Both tested the same substance, but the sensitivity of hs-CRP was higher and the lower limit of detection was lower. CRP testing is often used to assess the risk of infection or chronic inflammatory disease, and hs-CRP is more useful for assessing the potential risk of heart disease due to the detectable amount of CRP in bleeding. The whole blood hs-CRP is mostly dry chemical method, and the detection is more rapid. The serum hs-CRP is mostly analyzed by liquid phase chemical analysis and the detection linear range and sensitivity are wider and more sensitive than whole blood hs-CRP.
Wang Minyu, the head of the clinical biochemistry professional group of Qianfoshan Hospital, Shandong Province: This question involves the following two questions:
A, CRP and hs-CRP relationship?
CRP is usually detected by immunoturbidimetry, which is limited by the methodological method. The detection limit of the detection system is generally 5-10 mg/L, which is less than the range in the clinical laboratory. Xxmg/L reports to the clinic, and CRP is mainly used for the monitoring and evaluation of infectious diseases and autoimmune diseases.
With the deepening of CRP research, it is found that CRP below 5mg/L plays an important role in the risk assessment of cardiovascular and cerebrovascular diseases. Therefore, a more sensitive hs-CRP detection method is established to accurately The concentration of the specimen below the lower limit of CRP detection is detected.
B, the relationship between hs-CRP and serum hs-CRP?
Since both are detecting hs-CRP, that is, using different detection systems to detect the same substance. If the biological reference ranges of the two methods are consistent, then the results should be consistent; if the biological reference range is different, the results of the two measurements should be relevant.
Gu Guohao, Director of the First Affiliated Hospital of Suzhou University:
1. Relationship between serum Hs-CRP and serum CRP: Both the C-reactive protein (hs-CRP) and the C-reactive protein (CRP) actually measure the same substance, C-reactive protein (one of the acute phase-reactive proteins). The main difference is the measurement sensitivity and the measurable linear range. The specific relationship can be seen in the following figure:
2. Relationship between Hs-CRP and serum HsCRP in whole blood:Hs-CRP and serum Hs-CRP The commonality between the two is to detect the same protein of Hs-CRP, but the two types of specimens are different, and the detection principle is different. The whole blood hs-CRP detection principle is rate turbidity turbidimetry, and serum hs-CRP detection is immunoturbidimetry. The whole blood hs-CRP and serum hs-CRP results were in the range of low value < 5 mg/l and median 5-20 mg/l, and the correlation between the two results was good, and the results were very close. However, when the hs-CRP result is >20mg/L, the whole blood CRP result will be significantly lower than the serum CRP result. At this time, the detection serum hs-CRP is generally used in clinical diagnosis and treatment.